Composite

Part:BBa_K1660000:Design

Designed by: Dai Yuanyi   Group: iGEM15_BNU-CHINA   (2015-09-05)


bace16


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal XhoI site found at 151
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 247
    Illegal NgoMIV site found at 610
    Illegal NgoMIV site found at 682
    Illegal NgoMIV site found at 934
    Illegal AgeI site found at 541
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to have a better expression in E. coli, we optimised the coding sequence of rMpL, and add an L- arabinose induced promoter pBAD.


Source

It comes from the Macrolepiota procera.

References